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As depicted in Figure 2C, immunoblot evaluation of eluates with anti PfI2 antibodies reacted with one particular band at 20 kDa, corresponding for the migration on the recom binant PfI2 protein. Lane 3 confirmed the presence of His tagged PfPP1 by read more...

2 years ago

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How ever, the diploid strains containing PfPP1 and PfI2 or control plasmids were not able to develop. When stringent cul ture disorders had been applied working with SD LWHA medium, the strains containing PfPP1 PfI2WT, PfPP1 PfI2 or PfPP1 PfI2W16A read more...

2 years ago

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Enzyme activity was determined by spectrophotometric readings created at e citation and emission wavelengths of 360 nm and 460 nm, respectively, in endpoint mode, utilizing a SpectraMa M2 microplate reader. Calculations of net fluorescence were ma read more...

2 years ago

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Actin polymers also demand cor tactin, which stabilizes nucleation web pages for actin branch ing and elongation. Crip1 facilitates actin filament bundling and stabilizes actin interaction by using a actinin as well. Linkage of actin polymers read more...

2 years ago

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Mounting medium together with the nucleus specific fluorescent marker four,six diamidino two phenylindole, was used to preserve fluores cence. Eventually, the preparations had been e amined by transmission and fluorescence microscopy read more...

2 years ago

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E amin ation from the phosphorylation degree of Akt during the HAstV1 infected cells incubated with LY294002, wortmannin, triciribine, or MK2206 for 24 h showed that all but triciribine treatment successfully blocked the phosphoryl ation of Akt. A read more...

2 years ago

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As depicted in Figure 2C, immunoblot evaluation of eluates with anti PfI2 antibodies reacted with a single band at twenty kDa, corresponding to the migration in the recom binant PfI2 protein. Lane three confirmed the presence of His tagged PfPP1 f read more...